Polyhistidine | 蔬菜好營養
![Polyhistidine](https://i.imgur.com/4LfH9YV.jpg)
Apolyhistidine-tagisanaminoacidmotifinproteinsthattypicallyconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusof ...
![Polyhistidine](https://i.imgur.com/4LfH9YV.jpg)
Molecular biology technique
A simple gravity flow column for Ni2+-affinity chromatography. The sample and subsequent buffers are manually poured into the column and collected at the bottom end after flowing through the resin bed (in light blue at the base of the column).A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as a hexa histidine-tag, 6xHis-tag, or His6 tag. The tag was invented by Roche,[1] although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are commercially available from multiple companies.[2]
The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more H...
Hexa | 蔬菜好營養
His | 蔬菜好營養
His | 蔬菜好營養
His-Tag | 蔬菜好營養
His | 蔬菜好營養
Polyhistidine Tag | 蔬菜好營養
Polyhistidine | 蔬菜好營養
The impact of a His | 蔬菜好營養
![](https://i.imgur.com/4LfH9YV.jpg)
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