His-Tag | 蔬菜好營養
ProteinpurificationviaHis-tagisoftenreachedbyanimmobilizedmetalaffinitychromatographyorinshortIMAC.Thisisawidely-usedpurificationmethodto ...
The polyhistidine- or His6-tag is a protein tag originally developed for efficient protein purification in 1988 (Hochuli, Bannwarth & Döbeli et al., 1988). As a result of its comparably great cost-benefit ratio, it is known as one of the most widely used protein affinity tags to date. Due to its small size, variable between four to ten amino acids, it seldom interferes with the target proteins function. Additionally, its low immunogenicity and versatility under native and denaturing conditions are elevating this protein tag to stand out among others. The underlying purification principle of the His-tag is an interaction between metal ions and the imidazole ring of histidine.
The FeaturesTable 1: Overview of the most important aspects of the protein affinity tag
Features of the His-tag: ...His | 蔬菜好營養
His | 蔬菜好營養
Recombinant Protein | His | 蔬菜好營養
His-Tag Purification | 蔬菜好營養
His | 蔬菜好營養
Purification of His | 蔬菜好營養
His-Tag蛋白質偵測套組 | 蔬菜好營養
His Tagged Protein Purification | 蔬菜好營養
His-Tag | 蔬菜好營養
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